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The poly(A) tract is added posttranscriptionally to mRNAs in the nucleus in a two-step RNA processing reaction; a precursor RNA (or pre-mRNA) is processed at a specific site, and the processed RNA subsequently polyadenylated by a specialized nucleotidyltransferase, poly(A) polymerase.
However, the automation of multi-step sample processing, reaction and detection remains a great challenge.
The second element is a purine rich histone downstream element (HDE), which is removed during the processing reaction [18].
The processing reaction was carried out for 90 min at 37°C (Fig. 4A), or for the times indicated in Fig. 4B.
More recently, droplet-based microfluidics has become an increasingly powerful tool for reducing and processing reaction volumes ranging from microliters to nano-, picor, or even femtoliters.
Strand sequences, dye information, experimental methods, AFM images and methods, CanDo images, photobleaching correction processing, reaction rate calculations, and threshold window calculations.
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Many processing reactions including epitaxial growth, doping, oxidation, and silicidation are affected by the native defects in Si such as vacancies, self-interstitials, and their complexes.
All members of the Hedgehog family undergo a unique series of post-translational processing reactions [7].
RNA processing reactions have been extensively studied using biochemical systems; however, these are functionally linked in living cells providing increased efficiency and regulatory potential for gene expression [3].
LNA-tat-2 was designed to fully complement any siRNAs generated by three Dicer processing reactions that are 21-bases apart.
Nonetheless, by optimizing the particular arrangement of siRNA-encoding sequences along the lhRNA duplex, effective multiple targeting is possible for up to two Dicer processing reactions.
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