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All images were processed with the MRC image processing programmes to correct for crystal lattice distortion (Henderson et al., 1986).
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Image processing was performed using the MRC image processing programmes.
Natural language processing programmes have previously been found to function more effectively for variables that are narrowly and consistently defined.
To determine the lattice parameters, a projection image of a single-layered 2D crystal was calculated from the corresponding z-slices of the tomographic volume by first extraction and conversion into a projection image using the IMOD package (Kremer et al., 1996) and then processing the projection images using the MRC image processing programmes (Crowther et al., 1996).
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An automated sizing and counting approach based on Image J (a Java-based image processing programme developed at the National Institutes of Health) [1] has been performed for all near-bulk irradiation data.
Image analysis was performed using Image J software (a freely available Java image processing programme).
Data were acquired from 5000 cells, stored in collateral list mode, and analysed using the WinList processing programme (Verity Software House Inc., Topsham, ME, USA).
Accelerometry data were analysed using a batch processing programme (MAHUffe: http://www.mrc-epid.cam.ac.uk/Research/PA/Downloads.html) to remove data recorded after 23:00 and before 6 00.
A fully automated method, which attempts to mimic Cumulus, was recently developed by some of us (JL, KC, KH and PH) based on a public domain Java image processing programme, ImageJ (Li et al, 2012) (referred to as ImageJ-based method hereafter).
The eight areas of care were developed through an iterative process that occurred through face-to-face workshops, teleconferences, written feedback using Google Docs (a web-based word processing programme) and review of the research literature on consumer (patient and family) experiences with their care and goal setting.
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