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In addition, analyses of protein levels [ 5, 6] can ensure that engineered modifications are not affecting RNA processing or stability, and that post-transcriptional processes do not compensate for transcript levels.
This discrepancy could be due to differential mRNA processing or stability and/or regulation of the transgenes at the transcription/translation levels15.
This result not only provided additional evidence that the requirement for NELF is dependent on attributes of the flanking cis-regulatory DNA, but also rules out explanations based on differences in the processing or stability of the slp1 and lacZ mRNAs.
The difference of tissue expression profiles of uncoordinated miRNAs and the corresponding miRs* suggests a post-transcriptional regulation of their processing or stability.
If alteration of processing or stability happens to miR or its miR*, then their expression profiles may differ, leading to the corresponding low correlation coefficient values.
It is possible that elevation of tRNA levels could be a consequence of ppGpp-dependent differential processing or stability rather than direct ppGpp-dependent regulation.
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This review does not touch upon the processing, maturation or stability of the mt-transcripts or the process of turnover but these areas are also under intense investigation.
ASE is most likely the result of different allelic rates of transcription, transcript processing, or transcript stability.
Therefore, although increased precursor processing or miRNA stability cannot be ruled out, the observed elevation in mature miR455 upon FSK treatment of BeWo cells can be attributed to increased expression of the COL27A1 gene.
In contrast, both LND cells demonstrate markedly increased levels of processed NTF and CTF, indicating a markedly increased level of PS-1 proteolytic processing or proteolytic fragment stability compared with WT cells.
Possible explanations may be the use of different promoters, different post-transcriptional processing or differences in stability of mature miRNAs.
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