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The large dataset causes three limitations: (i) sorting and searching in a large dataset are time consuming; (ii) storage of the sorted background scores requires a large amount of hard disk space; and (iii) processing of the sorted array requires a great deal of memory, which is not usually feasible on a personal computer.
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Competition for the job of processing the sorted waste is coming not just from other waste companies but also from technology companies that think they can transform this trash into higher-value materials.
After sorting, we immediately reanalyzed samples of the sorted cells.
Batches with the highest purity of the sorted fraction (93 and 88% for 7BS and 7BL, respectively) were used for further processing.
Gene expression analysis of the sorted tumor cells revealed that the Aldefluor+ tumor cells has decreased expression of transporter associated with antigen processing (TAP) genes and co-stimulatory molecule CD80, which would decrease susceptibility to T cells.
In output the array index contains the indices of the sorted array.
The present study showed that 99.4% of the sorted cells displayed green fluorescence.
The suffix array consists of the sorted suffixes of a string.
The purity of the sorted CD31+CD45− cells was >98% (Fig. 4a).
Extended Data Fig. 3 Doublet composition of the sorted CD4+CD32high T cells.
The suffixes of the sorted elements form a sequence.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com