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Processing of the slides followed methods as previously described in [42].
No further processing of the slides was performed in GenePix.
IMMCO provided the funding to LS and KM for raw materials and time required for processing of the slides.
Amine coupling of the oligonucleotides to the slide surface and post processing of the slides was performed according to the manufacturer's protocol (Amersham Biosciences).
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Anaplastic lymphoma kinase IHC expression was non-informative in 67 (8.7%) TMA spots mainly because of the loss of tissue core during processing of the slide or because of the TMA spot being non-representative of tumour.
Post-processing of the slides included UV irradiation followed by 1 h incubation at 80°C in a vacuum oven.
The authors acknowledge Dr.Martin Villiger for the assistance with digital processing of the histology slides.
To distinguish the MIF optical absorption and absorption of light by silver nanoparticles formed in the bulk of the glass slides, we subtracted the spectra of the samples after the surface film removal from the spectra measured after the processing of the glass slides in hydrogen atmosphere.
The processing of slides, the preparation and storage of DNA samples, and the polymerase chain reactions (PCR) were performed in other rooms than those in which PCR products were manipulated.
Procedures for identifying neurons in skin biopsies employed immunofluorescence techniques in a modification of the protocol described by McCarthy et al. 13 The processing and cutting of the slides were done following procedures previously described.
Data processing of the scanned microarray slide included signal intensity measurement with the ImaGene software program, followed by median pin-tip (c.q. sub array) normalization.
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