The flow chart of the pre-processing of gene expression data is described in the upper-right corner of Figure 1.
We also demonstrated the tissue specificity of modules, based on a post-processing analysis of gene expression in additional tissues.
The GO term enrichment analysis of housekeeping genes show the enriched biological processes GO terms like "translation", "protein folding", "protein localization", "rRNA processing" and "regulation of gene expression" (Table S4).
GO terms like "translation", "protein folding", "protein localization", "rRNA processing" and "regulation of gene expression" indicate that most of these housekeeping genes are involved in regulation of transcription and translation.
LC contributed to sample processing and validation of gene expression.
NO contributed to microarray sample processing and validation of gene expression.
EM contributed to sample processing and validation of gene expression analysis.
SM contributed to microarray sample processing and validation of gene expression analysis.
Within-mouse variance captures variation due to RNA extraction, array processing, and heterogeneity of gene expression within tissues, which may be amplified by dissection and tissue collection procedures.
The number of methods for pre-processing and analysis of gene expression data continues to increase, often making it difficult to select the most appropriate approach.
Qi, L., Haurwitz, R.E., Shao, W., Doudna, J.A. & Arkin, A.P. RNA processing enables predictable programming of gene expression.
