FISH methods need specific sample handling, such as tissue processing and method of fixation, standardization of reagents and protocols, standardized reporting criteria, and implementation of quality assurance procedures, including the requirement for laboratory accreditation and external proficiency assessment [ 22].
Preanalytic factors, such as the processing of specimens, the fixation method, and the choice of antibodies also introduce variability [ 50].
Consequently, a split in foveal processing at the point of fixation would cause the exterior letters of fixated words to project to different hemispheres and valuable information provided by conjoint exterior letter features may be lost (see Footnote 4 in File S1).
Consequently, although a functional division in hemispheric processing at the point of fixation is fundamental to the split-foveal processing view, the mounting evidence obtained with appropriate experimental precision from Latinate and now Arabic languages provides no indication that such a division exists.
However, this asymmetry in word recognition was observed only for extrafoveal displays and no indication of a functional division in hemispheric processing at the point of fixation was observed [4], [29], [30].
Moreover, since it is well-established that the LH generally has superior perceptual capabilities for words than the RH (see [28] for a review), this putative division in hemispheric processing at the point of fixation is claimed to have important effects on word recognition [18] [27].
Additional controls of fixed and frozen brain tissues from the same source were used to assess the effect of fixation, processing, and retrieval on the biological properties of the TSE agents present.
The varied findings may be due to a number of reasons including a limited number of sampling sites in our material, differences in tissue processing such as length of fixation, and differences in average time interval between blast events and death that was somewhat longer in the case series of Goldstein et al [ 28].
The current formalin-based methods of fixation and processing of tissue for histopathological evaluation hinder the reliable analysis of macromolecules.
Only few studies have adequately addressed analytical aspects of fixation and processing on array CGH.
