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One idea is the requirement for SPARC in the post-translational processing of collagen, where it acts as a molecular chaperone to ensure its stability [38].
The latter are involved in post-transcriptional processing of collagen fibers and formation of the triple helices.
The present study has identified genes associated with the processing of collagen, other structural proteins, and disulfide-mediated cross-linking during eggshell membrane formation in the white isthmus.
A major substrate of prolyl hydroxylation is collagen and appropriate post-translational processing of collagen requires both C-P3H and C-P4H, respectively.
Therefore, adipocytes play a vital role in defining the ECM environment for normal and tumour-derived ductal epithelial cells and contribute significantly to tumour growth at early stages through secretion and processing of collagen VI [ 66].
Although MMP-2 is considered in most circles to function in the secondary processing of collagen, studies have shown its ability to cleave intact triple-helical collagen with some efficiency [ 5, 33].
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Those molecules may reach chondrocytes via free diffusion or may be bound to collagens or proteoglycans on extracellular matrix superstructures becoming available on metabolic processing of collagens and/or proteoglycans.
TGF-β stimulates the expression of both collagen I and the enzymes involved in posttranslational processing of collagens such as BMP-1/mTolloid proteinases and lysyl oxidases (Lee et al, 1997; Uzel et al, 2001).
Mammalian sFrp-2 has been found to enhance the function of the metalloproteinase Bmp1, which is a rate-limiting enzyme in the processing of pro-collagen during collagen fiber formation at physiological low concentrations (10 20 nM) [ 91] (Fig. 3, Wnt-independent route).
However, the processing of the collagen is tightly controlled by the suppliers' own quality management systems, and in our own experience we have found that results are reproducible between different batches.
Native CII is a fibrillar protein composed of three identical α1 II) chains derived through extracellular processing of pro-collagen.
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