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3) Variability of technical replicates (repeated processing of aliquots of the same RNA preparation) must be small so that differential expression measured between samples is most likely to represent biological differences between samples.
Results of processing of aliquots immediately after blood sampling and after seven hours of courier transportation were compared by paired t-test.
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Table 4 summarizes the recovery of LV vectors following processing of 500-ml aliquots of HYPERFlask vessel-derived supernatants.
The inter-and inra-assay precisions of the extraction and chromatography procedures was evaluated by processing replicate aliquots of drug-free sheep and goat plasma samples containing known amounts of the drugs on different days.
The inter-assay precision of the extraction and chromatography procedures was estimated by processing replicate aliquots (n = 6) of pooled sheep plasma samples containing known IVM concentrations (0.1-2.0 0.1-2.0-40 ng ⁄ mL) on different working dand.
At times additional processing of samples, such as weak cation exchange (WCX -extraction, required refreezing of aliquots.
For processing assays, aliquots were divided into two and supplemented with either purified rPfSUB1 or an equal volume of control buffer (10 mM Tris HCl pH 7.6, 150 mM NaCl and 10% glycerol).
Thawing of aliquots was allowed only once.
The similarity between the two technical replicates demonstrates that aliquot production from the master library, processing of the samples as well as sequencing and analyses are highly reproducible.
The processing of information underwent revolutionary change.
Processing of information requests 14.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com