Sentence examples for processing in vitro from inspiring English sources

In order to test whether the proteasome can directly process Def1, we reconstituted proteasomal processing in vitro using Def1 and 26S proteasome purified to virtual homogeneity.

Characterization of fragments produced during mt-like tRNA processing in vitro and in vivo suggests that these RNAs are not fully processed at their 5′ ends and are cleaved internally.

Purified LIN28A inhibits pri-let-7 processing in vitro and its ectopic expression selectively blocks pri-let-7 processing in vivo (Newman et al., 2008; Viswanathan et al., 2008).

Vaccine-induced antibodies also inhibited parasite growth and interfered with antigen processing in vitro.

For microarray processing, in vitro activated, FBR and blood CD68+ cells were analyzed in triplicate on Rat Genome 230 2.0 Array GeneChips (Affymetrix, High Wycombe, UK) by a specialized microarray facility (DNAVision, Gosselies, Belgium) according to the manufacturer's double-round T7-based amplification protocol (Affymetrix).

Endoplasmic reticulum (ER) stress has been shown to be associated with neurodegenerative disorders including AD [7] and the ER chaperone protein, glucose-regulated protein 78 (Grp78) was demonstrated to bind APP and modify APP processing in vitro [8], [9], and was found in a complex with APP in vivo [10].

Here we show that insulin is a much less effective amyloid inhibitor of both IAPP and proIAPP processing intermediates in vitro in the presence of model glycosaminoglycans, but does inhibit the formation of amyloid by proIAPP processing intermediates in a homogeneous solution.

It has been shown that at least liver cells may get necrotic, when too many shRNA molecules interfere with the cellular microRNA processing machinery in vitro [19] [21] and in vivo [22].

This conclusion is consistent with its low tRNA processing efficiency in vitro [19] and with the fact that its gene (ELAC1) is part of a homozygous deletion in a lung cancer cell line, and thus apparently dispensable for cell survival and growth [20].

Furthermore, the effect of processing and in vitro or in vivo digestion on the release and availability of peptides with bioactive sequences is evaluated.

The RNA preparations were subjected to TEX digestion before cDNA synthesis and, given in vivo transcript processing and in vitro fragmentation during purification, the resulting cDNA libraries were, as expected, enriched for 5′-terminal sequences.