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It is important to remember that the waste amounts reported in the table could be temporary, as it is possible to decrease the waste by processing further orders.
Following fixation, the tissue was processing further on a Miles Scientific Tissue-Tek VIP automated processor.
Each sample was then stored at −80°C until processing further on microarrays.
The main factors determining the time taken for the TVC to reach these levels are the initial count due to contamination during slaughtering and processing, further contamination during storage, temperature, pH and relative humidity.
Prior to processing further, all fractions were immediately cooled in an ice water bath at 4°C and 100 μg/ml phenyl methyl sulfonyl fluoride (PMSF), 10 μg/ml leupeptin, 10 μg/ml pepstatin, as well as 10 μg/ml antipain (all acquired from Sigma, USA) were added to each plastic heparin tube before the blood samples; these additions served to inhibit proteases.
The in-line SFV particle size data, obtained every 10 s during processing, further allowed to explain and better understand the (in)significance of the studied DOE variables, which was not possible based on the LD data as this technique only supplied end granule size information.
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The following paragraphs will explain the processing steps further.
The processing sector further suggests that ports where processing historically took place should be given precedence.
The new programs take word processing a step further.
Contigs longer than 1,000 bp were used for further processing.
Images were imported into ImageJ for further processing.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com