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Gonads and internal organs were removed before processing for isolation of MEF cells.
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In solid tumours, the tissue processing required for isolation of single cells is more challenging.
Unlike gold and silver, which could be readily isolated in a comparatively pure state by simple fire refining, the platinum metals require complex aqueous chemical processing for their isolation and identification.
Internal organs and gonads were removed before processing for cell isolation.
The serum samples were stored at −80°C until further processing for exosome isolation.
The serum fraction was then frozen at −80°C until processing for DNA isolation.
Colleagues have inquired regarding specific details of our approach to MG whole mount preparation, cryosectioning, and processing for LCM and RNA isolation.
The ticks in our study were not held for any length of time to allow for blood in the ticks to be digested before processing for virus isolation.
However, selection of the antigens of interest, methods for introducing TAAgs into MHC class I and II processing pathways, methods for isolation and activation of DCs, and route of administration are the parameters to be considered for designing and conducting clinical trials with engineered DCs.
Specimen processing and culturing for isolation of mycobacteria was carried out following standard procedure described by OIE [ 12].
Sample processing and culturing for isolation of mycobacteria was carried out at the mycobacteriological laboratory at the National Veterinary Institute, Norway following the standard procedure described in the manual of diagnostic tests and vaccines for terrestrial animals [ 18].
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