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All 6,860 unique Affymetrix H. glycines gene sequences (contigs) were aligned against the non-redundant GenBank database (downloaded November 2005) using the following parameters in BLASTX with a post-processing cutoff value of 1e-05: filter = seg, lcfilter, W = 4, T = 20, E = 100, B = 25, V = 25, topcomboN = 1, golmax = 10.
For Additional data file 1, all H. glycines parasitism gene nucleotide sequences (as found in GenBank) were aligned against the non-redundant GenBank database using standard NCBI BLASTX parameters with a post-processing cutoff value of 1e-15 (dated March 2008).
To analyze cross-hybridizing G. max and P. sojae probesets, the respective Affymetrix probeset nucleotide sequences were aligned against the non-redundant GenBank database (downloaded December 2005) using the following parameters in BLASTX with a post-processing cutoff value of 1e-10: filter = seg, lcfilter, W = 4, T = 20, E = 100, B = 10, V = 10, topcomboN = 1, golmax = 10.
Therefore, the estimated frequencies representing the heart and the respiration rates are each lowpass filtered with first order infinite impulse response (IIR) Butterworth filters, designed with the Matlab R2011a (MathWorks) "Signal Processing Toolbox" with cutoff frequencies of 0.1 Hz and 0.05 Hz, respectively.
To facilitate data processing, we established a cutoff for GO terms that were present in only one sequence within the DAG generated (data not shown).
We reanalyzed their microarray data (four Arabidopsis ATH1 microarrays, two time points, no biological duplication) with the same data processing methods (1.2-fold cutoff threshold) used in this study.
St. Lucie County did not meet a noon cutoff to finish processing 37,379 ballots that were cast early in the 18th Congressional District race, but it eventually released the results, which showed Mr. Murphy gaining votes in the recount.
Note that length values in vitro and in silico are not comparable – the FilaQuant software processing florescence microscopy images has cutoff parameters (regarding length and thickness of lines to consider a filament) while for the simulation every integrin/focal adhesion with at least one bound actin is considered a filament.
GPS data from October 2008 to November 2009 were analyzed by the GIPSY/OASIS II software using the Precise Point Positioning (PPP) processing strategy for five different elevation cutoff angles from 5° to 25°.
In that case, the user must select a cutoff under the weight processing parameters.
In the absence of a "gold standard" for processing microarray data, these statistical threshold cutoffs serve to maintain a reasonable and consistent level of data quality across all studies analyzed within NextBio and are commonly adopted in the literature [43] [45].
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