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We then attempted to identify novel miRNAs from our four sequenced RNA samples separately using the precise processing criterion as described in methods.
We used the same data processing criterion of a recent study from Van Sluis et. al. [ 42] and children who did not reach 500 minutes collected on at least 2 weekdays were excluded from the school-time analysis.
Our result further demonstrated that such a precise processing criterion [ 36] could be used as a straightforward and reliable method to identify the miRNA from the diverse small RNA data.
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The dataset contained raw sequences, for which the pre-processed sequences passed the preAssemble [ 11] processing criteria of sequence quality and vector contamination (Construction and content).
Data were downloaded using Actigraph software V.3.8.3 (Actigraph, Pensacola, Florida, USA) and subsequently processed in-house, 21 according to predetermined processing criteria.
Several post processing criteria are defined to filter false positives.
The information gathered may shed light on the design and processing criteria of bioactive bone scaffolds.
Among all sources of uncertainty, such as data processing, criteria selection, and threshold determination, criteria weight is regarded as the greatest factor contributing to the uncertainty of AHP.
It is important to select appropriate data processing criteria.
Discrepancies among the reported frequencies might be due to different factors, including distribution of histological types, tissue processing, criteria for the scoring and interpretation of the staining results.
In this work, we describe a workflow involving sequence ladder and improved signal processing criteria on a large MS/MS dataset exemplified in the MS Cleaner version 2.0 that efficiently reduces the number and the size of spectra and, subsequently, dramatically shrinks the computing time used by the interpretation software.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com