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Taken together, the obtained data identify DALIS as an antigen processing compartment that contains a distinct set of proteostasis factors.
In mammals these receptors allow efficient delivery of antigen to the processing compartment via receptor-mediated endocytosis [22].
It has been postulated that the immunogenicity of a protein antigen can be enhanced by targeting the antigen to the major histocompatibility complex type II (MHC II) processing compartment of professional antigen-presenting cells (APCs).
One of the approaches is the use of the lysosomal associated membrane protein 1 (LAMP) as a LAMP/antigen chimera to target endogenous antigens to the MHC II processing compartment [5] [10].
To further explore the role of macropinocytosis in ovalbumin uptake and processing, DQ-ovalbumin was used as it has intrinsic fluorescent properties after exposure to a processing compartment.
They emerge from an intracytoplasmic membrane complex known as multivesicular bodies, which can be understood as a processing compartment for internalized proteins, subjected to the influence of the trans-Golgi network.
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DALIS were previously proposed to act as antigen storage and processing compartments to coordinate DC migration and antigen presentation [29], [32].
Our finding that lens fiber cell Aβ is exclusively cytoplasmic suggests that this amyloidogenic peptide may be released from intracellular processing compartments during organelle disintegration accompanying epithelial-to-fiber cell terminal differentiation.
B cells specific for a particular antigen have an exquisite capacity to concentrate and direct the antigen to the appropriate processing compartments.
However, anti-HER2/ neu antibody-cytokine fusion proteins such as IgG3- IL-2) redIgG3- IL-2traffIgG3- IL-2ECDHEredirecttheen processing compartraffickingendritic cells resulting in effective antigen presentatiof.
It appears as if plants have generally abandoned RNA-based RNase P and make use of the proteinaceous enzyme type in all their tRNA processing compartments (see also Section 2.1).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com