For instance, possible real-time reversible pore-size changes during ultrasound propagation through skin/leather matrix could be a reason for diffusion rate enhancement in leather processing as reported for the first time.
Considering the localization of PTP1B at the cytoplasmic face of the ER [ 36], reduced PTP1B levels in Nck1-depleted cells might induce activation of the PI3K/Akt pathway in a ligand-independent manner by promoting phosphorylation of newly synthesized RTKs during their processing, as reported for the insulin receptor precursors at the ER [ 37].
Sample collection and processing were as reported previously [ 34].
Evoked KCs are suggested to originate from a brain state with relatively increased stimulus processing, revealing an activity pattern resembling novelty processing as previously reported during wakefulness.
Total RNA was extracted using the RNeasy kit (Qiagen, Hilden, Germany), with quality control and method for data processing as previously reported (Engelsen et al, 2008a; Salvesen et al, 2009).
All the new blocks (TMAs) were stored at 4°C, before sections 4- μm thick were prepared for each marker to be examined by immunohistochemistry. Serial tissue sections were prepared and stored at 4°C for 24 h before immunohistochemical processing, as previously reported (Garcia et al, 2007a, 2007b).
It is important to understand if functional connectivity abnormalities in this network could be correlated to minimal impairments in neuropsychological performances, such as processing speed, verbal memory, as reported in migraine in interictal attack period.
Sampling, cell fixation, hybridisation and image processing were carried out as reported earlier [5].
We employed a pre-processing and normalization pipeline as reported previously.. Color-bias adjustment, quantile normalization, probe type bias adjustment and batch correction were performed.
At the end of each treatment time, cells were collected after a brief incubation with trypsin EDTA followed by processing for cell cycle analysis as reported earlier [ 14].
Second, immobilization of the rat eye could be improved by designing a user-friendly system as already used in confocal microscopy; possible artifacts due to fast eye motion could be also corrected by use of suitable image processing to reconstruct undistorted images as reported for confocal microscopy [ 38].
