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These ubiquitous inhibitors of Cys proteases play several important roles in plants, from the control of various physiological and cellular processes in planta to the inhibition of exogenous Cys proteases secreted by herbivorous arthropods and pathogens to digest or colonize plant tissues.
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They should be ideally suited to investigating infection processes in-planta and could be used to investigate aspects of infection/plant pathogenesis, by B. cinerea and are particularly suited to the detection and quantification of the pathogen prior to the development of symptoms.
Accordingly, both the TPSs were coexpressed with HMGR in planta to observe the production of sesquiterpenes.
It has also been reported that the plant growth regulator indole-3-butyric acid is activated in planta to indole-3-acetic acid (IAA) by a process similar to β-oxidation [ 59].
The T-DNA is transcribed in planta, to generate biologically active viral RNAs that can initiate self-replication.
Xylogenesis has led to important discoveries in the understanding of xylem formation in planta, and genes in common between the xylary processes in planta and in vitro have been found (Miyashima et al., 2013).
To extend our in planta observations to the single cell level, inoculation experiments were also performed using Arabidopsis protoplasts.
Our data demonstrate that the amiRNA precursor is efficiently processed in planta in all tissues tested and the amiRNA-guided cleavage of target transcripts is highly specific.
A sequence of specific cellular events involving cell differentiation is required to form a mature TE and the study of this process in planta is difficult because differentiation occurs within complex tissues in the plant body.
The agroinfiltrated fruits continued the developmental process in planta.
The expected amiRNA species were readily detected by RNA blot in every line and tissue tested, which demonstrates that the amiRNA precursor is efficiently produced and processed in planta (Figure 3).
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