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Membrane processes for the isolation of serum proteins from whey, and the reduction of bacteria and spores in skimmed milk have already been adopted by the dairy industry.
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In this contribution the Product-driven Process Synthesis methodology was used as a well-defined structured approach for the conceptual design of an extraction process for the isolation of intact oil bodies from soybeans.
Study design: The lens aspirates collected during the 9-year period (from 1990 to 1998), from 70 children up to 12 years of age with congenital cataract were processed for the isolation of rubella virus by conventional viral isolation culture method using BHK-21 and Vero cell lines.
In parallel, blood samples were immediately processed for the isolation of Peripheral Blood Mononuclear Cells (PBMCs) by density gradient centrifugation using Ficoll-Hypaque (Sigma).
The sorted GFP-expressing cells were processed for the isolation of RNA.
This was centrifuged, and cell mass was further processed for the isolation of enzymes separately.
All the blood was processed for the isolation of plasma or serum within 4 h of collection.
Four mL of heparinized venous blood sample was collected from the umbilical cord just after delivery of the neonate and processed for the isolation of erythrocytes and plasma.
Whole blood samples (3 mL) were collected in admission and processed for the isolation of serum within 4 h of collection.
Tissues collected were fixed in 4% (w/v) paraformaldehyde, flash-frozen in liquid nitrogen, or processed for the isolation of mitochondria.
In addition, the cell lysis and tissue processing solutions used for the isolation of DNA should be examined for their contribution to excessive chemical noise, and the DNA isolation process could be modified by including additional DNA purification after isolation.
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