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These results suggest that treatment with menadione activates antioxidant defense mechanisms responsible for GSH production, or recycling, or by both processes, as previously published [25].
The mRNA expression of Sprouty4 coincides with regions of FGF signaling activity in the mouse embryo at embryonic day (E) 10.5, including the PSM, the somites, the limb buds and the frontonasal processes as previously reported (Figure 1A) [27], [27].
After anaesthesia (chloral hydrate, 400 mg/kg, i.p). the limbs and the back were shaved and tattooed in order to locate the bony processes as previously described in details [15].
These women complete all the screening processes as previously described and have to meet the ICD-10 criteria for depression.
However, they displayed an activated morphology with a prominent cell body and elongated processes as previously described [ 15] (Fig. 3b).
This multiphasic food response may reflect trade-offs between multiple food-regulated processes as previously discussed (Kaeberlein et al., 2006; Lee et al., 2006).
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Lysates were prepared from E12.5 total hindbrains and then processed as previously described [18].
Lysates were loaded onto SLIDE and processed as previously described.
Sequencing data was processed as previously described17,63.
WGBS libraries were processed as previously described60 (see Supplementary Methods).
RNA-seq datasets were processed as previously described16 (see Supplementary Methods).
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