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In total, 96%% of oligosaccharides on CHOrFVII were core fucosylated, bi-antennary structures carrying no, one (35%%) or two (57 %) sialic acid residuesor incompletely processed variants.
VEGF-B, another distinct member of the VEGF family, is expressed in the two differently spliced and proteolytically processed variants VEGF-B167 and VEGF-B186 [ 9– 11].
As described for SmelA075, SmelA060, and SmelA072 in S. meliloti, and GadY in E. coli, processed variants of non-coding transcripts are not unusual in bacteria [ 17, 61].
Besides the described mRNA variants indicated in Table 1, many more processed variants for the majority of the genes were predicted from the analysis of EST (Expressed Sequence Tag) and SAGE (Serial Analysis of Gene Expression) data.
The remaining 15%% were shown to be incompletely processed variants, hybrid-type structures with and without sialylation and antennary fucosylation, and high-Man structures on the light chain, which were not detected on pdFVII, or the other rFVII preparations.
In addition, the supernatant of a glucose-based culture showed two bands on acrylamide gel corresponding to the expected sizes of the two different processed variants of the protein, 45 and 47 kDa (Fig. 2), as it has been previously described [ 27].
Similar(54)
It judges process variant profiles according to their value contributions, measured in terms of the risk-adjusted expected NPV of the process cash flows.
We extended the versatile C++ class library by adding functionality to import and process variant call format (VCF -based file formats used in DNA sequencing and SNP calling.
Two catalytic reforming process variants (i.e. processing same inputs and producing same outputs with different internal unit operations arrangement; for this reason, these process variants have the same function) are compared from these previous different points of view.
Moreover, process variants must be split into context-specific process variants and a standardized master process.
Moreover, process variants must be split into context-specific process variants and a standardized master process. .
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