Sentence examples for processed to total from inspiring English sources

Two D and 3D cultures of MCF-10A cells were processed to total cellular lysates and analyzed by Western blotting as described previously [ 25].

To quantify mis-spliced enosf1b following e10i10 MO injection, single standard control injected or e10i10 MO-injected embryos were processed to total cDNA using a scaled down version of the Trizol protocol outlined above.

K562 and Ba/F3 cells were processed to total cellular lysates by lysing in normal lysis buffer (50 mM TRIS/HCL pH 7.4, 1% Triton-X 100, 137 mM NaCl, 1% Glycerin, 1 mM Natrium Orthovanadate, 0.1 μg/μl Aprotine, 0.01 μg/μl leupeptin, 1mM AEBSF) and analyzed by western blotting as described previously [ 18].

At 12 hours post-infection, total DNA was extracted and processed to detect total viral DNA synthesis by submitted to semi-quantitative PCR analysis using LTR-Gag primers, as described previously [50].

Tumors were homogenized and processed to obtain total fractions for western blot as described previously [41].

Tissue samples were processed to extract total RNA and transcribed into cDNA.

The cells have been then processed to prepare total RNA and protein extracts.

Briefly, samples were processed to determine total aerobic bacterial count by standard Pour Plate technique.

The tumor tissues were processed to isolate total RNA and cDNAs prepared as previously described [ 6- 9].

Following incubation with pUC19 plasmid DNA, samples of Krebs-2 tumor ascites were processed to isolate total DNA.

Following RNAprotect (Qiagen) treatment to prevent RNA degradation, cells were lysed and processed to isolate total RNA as described in the instructions of Qiagen RNeasy midi kit (Qiagen) in combination with DNase I (Qiagen) treatment.