Celase GMP is a proprietary enzyme containing collagenase, neutral protease, and buffer salts that are produced using avian and mammalian tissue-free raw materials, and are aseptically processed, sterile-filtered, and highly purified under good manufacturing practices (GMP) guidelines.
Membranes were processed under sterile conditions, as previously described by Tseng et al. (1998).
The fat was aspirated using a 14-gauge three-hole blunt cannula, and then processed under sterile conditions for subsequent grafting into nude mice within two hours of its collection according to previously published protocols [28], [29].
To prevent contamination, all solutions were sterilized by filtration through a 0.2-µm membrane prior to γ-irradiation and they were processed under sterile cell culture conditions at all time.
Fresh tumor specimens were processed via sterile mechanical dissection.
Following tissue recovery, the samples were processed under sterile conditions.
The samples were processed under sterile condition using the Laminar flow hood.
Components of the bone marrow aspirate from the two iliac crests were processed under sterile conditions.
All samples were collected and processed under sterile and pyrogen-free conditions.
All samples were collected and processed under sterile, pyrogen-free conditions and were incubated at 37°C in a 5% CO2 saturated humidified incubator.
Primary human liposarcoma tumor samples of at least 1 cm3 were harvested intraoperatively from patients undergoing resection of an already diagnosed liposarcoma and immediately processed under sterile conditions.
