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In Experiment 2, oocytes were obtained by manual aspiration from the ovaries of slaughtered crossbred beef heifers (under 30 months, n = 1241) and cows (over 4 years old, n = 1125), and processed in vitro as above.
RNA samples were processed in vitro to generate libraries of short inserts, using the DNA Colonies Template Library system.
Mutagenic dI lesions processed in vitro can be scored by restriction enzyme cleavage to evaluate the repair.
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Since an ideal nutrient supply plays a major role during the initiation and maintenance of cell differentiation processes in vitro, a dynamic cultivation could be beneficial.
Modelling human disease processes in vitro is important for our understanding of the risks associated with human exposure to known and unknown inhaled chemicals or toxicants.
These cells often displayed extensive processes in-vitro and might therefore be particularly susceptible to damage during mechanical dissociation, especially given that the DG neurospheres had a tighter structure and were thus more difficult to dissociate compared to vSVZ neurospheres.
Adjacent sections were processed for in vitro autoradiography to assess the hippocampal distribution of TSPO and GR.
Tube formation assay demonstrates that ETV2 initiates morphological changes resembling endothelial process in vitro.
Controlling this process in vitro would represent an important advance for tissue engineering.
The resulting conjugate retained epitope generation ability upon binding to dendritic cells and enabled monitoring of intracellular processes in vitro and in vivo.
Rather than do it all in a dish, the group decided to try starting the process in vitro but completing it in an animal (in vivo).
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CEO of Professional Science Editing for Scientists @ prosciediting.com