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The samples were processed in triplicate to evaluate the reproducibility of sample processing and then analyzed in triplicate by nano-LC off-line MALDI TOF as described above.
Thyroids were removed 24 h after injection; total RNA was extracted from pooled thyroids and processed in triplicate using Illumina MouseRef-8 Whole-Genome Expression Beadchips.
The RNA samples were processed in triplicate at the Swegene Center for Integrative Biology Genomics DNA Microarray Resource Center (SCIBLU, Lund, Sweden) using MouseRef-8 Whole-Genome Expression Beadchips (Illumina, San Diego, CA, USA).
As we prepared two biological replicates with each processed in triplicate for immunoprecipitation and LC-MS/MS analysis, proteins that are quantifiable in at least two out of the three experiments of each biological replicate were retained for comparative analysis.
Samples were generated and processed in triplicate.
Samples were processed in triplicate according to the manufacturer's guidelines.
Similar(32)
Samples were processed in triplicates and every PCR run was repeated at least 2 times.
The OS region samples were processed in triplicates to demonstrate replicable results.
Repeated trials where the same specimens were processed in triplicates (NanoOrange alone, immunofluorescence alone, NanoOrange and immunofluorescence combined) and analyzed for all parameters with CellProfiler did not reveal any significant interference (data not shown).
Different genotypes were processed in biological triplicates and luminescence was measured using a Chameleon plate reader (Hidex, Turku, Finland).
Briefly, TACO is based on a systematic collection of samples of processed food, in triplicate, held in 9 cities of five geopolitical regions in Brazil (North, Northeast, South, Southeast and Midwest).
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