Consistency of staining as well as reduced bias during CyTOF runs are also improved as each sample is processed in the same tube at the same time.
Each frame has the same number of MBs, and we assume each MB has to be processed in the same amount of time.
Additionally, we used tissue microarrays for the immunohistochemical analysis: all tissue samples were automatically processed in the same conditions and at the same time in our pathologic laboratory.
Plasma protein profiles were collected in three batches, which we refer to as Studies A, B and C. All 3 of these studies were processed in the same laboratory but at different times.
Targeting efficiency using ZEN can be excellent, although there are significant variations among experiments we performed: 29% NHEJ mutation rate in one experiment (2/7), 100% in another (6/6), processed at the same time and under the seemingly identical conditions.
After several rinses in KPBS, sections were processed for the same time (10 min) in 0.04% 3-3′-diaminobenzidine-HCl and 0.006% hydrogen peroxide diluted in KPBS.
Five of ten cultures processed at the same time in the first instance and three of the ten in the second were submitted for RFLP analysis; they matched a strain used as a control in the decontamination and digestion procedure.
Samples were carefully transported frozen to be processed at the same time, in random order, by investigators blinded to the clinical data to avoid bias and batch variations.
To determine genotyping error in the GoldenGate bead assay, nine individual samples were run in duplicate on plates processed at the same time.
