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Each DNA sample was processed in duplicate, and each processing run included samples for background (reaction mixture with all components except SssI enzyme), a hypomethylation control (HeLa cell DNA), and a quality-control sample (DNA extracted from a whole-blood sample) to determine the inter- and intraassay CVs (1.8% and 5.3%, respectively).
Relative matrix effect (RME) was evaluated using six lots of human K3EDTA plasma including one hemolyzed and one lipemic plasma lot, processed in duplicate samples at LOQQC and HQC levels and the area ratio (i.e., peak area response of LS/peak area response of IS) was used to check the acceptability of the result.
All samples were processed in duplicate.
There was also excellent agreement between samples processed in duplicate.
To guarantee the reliability of the results, all samples were processed in duplicate.
For each drug (alone or in combination), three to four independent experiments were performed and all samples were processed in duplicate.
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Samples were processed in duplicates and diluted with PBS (1 100) before analysis.
Samples and standard curves were always processed in duplicates.
None of the patients included in the study had been exposed to HCV treatment previously to the interview, and a false negative PCR result is unlikely since the HCV-RNA detection technique has high sensitivity [33] and all samples were processed in duplicates.
Samples were diluted 1 10 and processed in duplicates.
All the samples were processed in a duplicate assay.
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