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Three days after incubation, cells were processed for the analysis of cell viability.
In addition, blood samples from 8 Boxer (Box) dogs were processed for the analysis of a subset of OR genes.
Cultured vein sections were processed for the analysis of proliferation (phosphorylated-histone H3 and Ki67) and apoptotic (cleaved caspase-3) markers, in addition to TUNEL staining.
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At 35 days of culture, the cells were then processed for analysis of the nature of the mineral phase deposited by all cell lines, including cells expressing mutant Ank molecules.
This allows the use of metabolic modelling as routine process for the analysis of expression data.
In the present study, the newly established UHPLC method was applied to a recombinant antibody Escherichia coli fermentation process for the analysis of total free amino acids.
Subsequently, the cells were collected by trypsinization and processed for the flow cytometric analysis of caspase-3 activation according to the manufacturer's instructions.
In each experimental group, six replicates were cultured in parallel, four were analysed histologically and two were processed for mRNA analysis of the SFB layer and cartilage.
The next day, 100 μm-thick sections were cut with a vibratome and processed for TEM analysis of the lateral and third ventricles, choroid plexus, SCO and SFO as described above.
Then, the specimens were processed for morphometric analysis of bone loss.
10 g of sediment per sample was processed for analysis of three hundred pieces of organic matter.
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