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Corneal limbal tissue of 2 mm3 (n = 6) was collected from the cadaveric donor eye from Eye Bank of medical research foundation, Chennai and processed for further experiments.
Since cyanide is a toxic compound that would affect/inhibit the microbe e.g. yeast fermentation behaviours, this simple pretreatment way claims to be an efficient method for detoxifying the biomass for being processed for further biological conversion, e.g. bioethanol/biobutanol/lactic acid production through fermentation.
Fractions were acetone-precipitated, and processed for further analysis.
Experimental and control animals were sacrificed after 48 hours, kidney biopsies were harvested and processed for further analysis.
Following imaging, the membranes were processed for further immunolabeling using an antibody re-probing kit (Chemicon, Temecula, CA).
The length of the colon was measured, the colon was flushed with cold PBS and opened longitudinally for morphologic studies, and tissue obtained from each colon was processed for further assays.
Dissected adult organs or embryos were immediately observed under a standard fluorescence stereomicroscope for GFP expression in transgenic animals and/or processed for further morphological analysis or placed in RNAlater solution (Ambion) and stored at −80°C for subsequent RNA extraction.
Dissected tumors are processed for further analysis.
The colon tissue was processed for further analyses.
After collection, blood samples were immediately processed for further experiments.
Tumors were collected from euthanized mice and processed for further analyses.
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