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Tissue samples were taken and fixed in 10% formalin solution, then processed for embedding in paraffin wax.
Kidneys were immersion-fixed in 10% neutral buffered formalin, and processed for embedding in glycolmethacrylate.
Fixed cells were processed for embedding in Epoxy, TAAB 812 Resin (TAAB Laboratories, Berkshire, England).
Samples were dehydrated in acetone and finally processed for embedding in Lowicryl K4M (Polysciences Europe, Eppelheim, Germany) [27].
Whole kidneys were photographed using a Lieca EZ4D dissecting microscope and processed for embedding in paraffin wax and sectioned at 5 µm.
For paraffin-embedded sections, freshly excised tissues were fixed in Glyo-Fixx (20% ethanol, 5% glyoxal, 1% propanol and 1% methanol) for 48 hours at room temperature and processed for embedding in paraffin blocks for sectioning.
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And they were processed for paraffin embedding for histological observation.
PtK2 cells were infected for 30 min, fixed and processed for EPON embedding.
For electron microscopic evaluation of myelin, mice were perfused as above and processed for resin embedding.
Human lungs were placed in 4% (w/v) paraformaldehyde after explantation, and processed for paraffin embedding.
Fragments of lungs were fixed in 10% neutral-buffered formalin and routinely processed for paraffin embedding.
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