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Tissues were processed for assessment of motoneurons, ventral root (VR) axons, and NMJs.
If fewer than 20 eggs were laid in each dish then all were processed for assessment of early stage embryogenesis.
Hematocytes were withdrawn from the hemocel cavity and immediately processed for assessment of lysosomal membrane stability and DNA integrity.
Because samples from the antagomir-treated animals were processed for assessment of expression of mRNA, not miRNA, we were unable to directly confirm the effects of treatment with antagomirs on the levels of miR-126.
Samples were centrifuged at 2,500 rpm for 10 min to remove ethanol by aspiration without disturbing the sperm pellets and processed for assessment of DNA damage according to TUNEL protocol (APO-DIRECT™ kit, BD Pharmingen).
The eggs that were not processed for assessment of early stage embryogenesis were incubated on cotton wool, and after 8 days of incubation, the number of eggs in each clutch with visible eyespots was counted, as well as the number that subsequently hatched.
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Embryos were then recovered from the agar plates and processed for TAG assessment or RNA extraction.
The recorded dEMG needed to be processed for reliable assessment of the respiratory neural drive.
At the time of sacrifice, the lower right lung lobe was infused in situ with 10% neutral-buffered formalin and preserved for 48 hours until processed for histopathological assessment.
At the end of the study, paws were removed and processed for histological assessment.
At the end of the study, paws were removed and processed for histopathological assessment.
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