In this study, we have examined the effects of specific chemical modifications of amino acid side-chains on the in vitro degradation of "native" collagen obtained from acellular matrix (ACM -processed ACM -processed
To validate the interpretation of OPT reconstructions following tomographic imaging, the same arteries were processed for histological examination.
Arteries were processed either as whole mounts or were sectioned at 10 μm.
Briefly, iliac arteries were processed with distilled water for 72 h at 4°C, 4% sodium deoxycholate for 4 h, and 2,000 kU deoxyribonuclease I (DNase-I) in 1 M NaCl for 3 h.
Two 2 mm thick transverse segments were cut from each artery at the level of injury in the femoral artery and processed for paraffin embedding.
Villagers eat more processed food—India's artery-clogging pudding, gulab jamun, now comes in packets, made in small factories in nearby towns.
Rats were anaesthetized as above and mesenteric arteries from control and diet-induced obese animals dissected after perfusion fixation (1% paraformaldehyde, 3% glutaraldehyde in 0.1 mM sodium cacodylate buffer with 10 mM betaine, pH 7.4), with short segments of artery being processed for electron microscopy [46].
Half the artery was processed for mRNA analysis and the other half fixed in 4% paraformaldehyde overnight for cryopreservation in Optimal Cutting Temperature compound (OCT).
Then, the rats were anaesthetized by using 20% urethane (1.2 g/kg intraperitoneally), and plasma (8 10 ml per animal) was immediately collected from the femoral artery and processed into serum.
The rats were then anaesthetized by using 3% pentobarbital (30 mg/kg intraperitoneally), and plasma (8 10 mL per animal) was immediately collected from the femoral artery and processed into serum.
Left kidneys were immediately processed to isolate renal arcuate arteries.
