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However, the method described here relies on independently measuring internalization and phagosomal acidification of internalized particles under identical experimental conditions; thus the process of internalization can be easily separated from phagosome acidification allowing for a more accurate measure of phagosome acidification rates.
Subsequently, they are internalized and assimilated into the mycobacterial cytoplasm and this process of internalization involves the interplay of IrtB-Rv2895c importer system.
The magnetic moment and reduction in size of Fe2O3 nanoparticle seems to be the key factor in the process of internalization and phagocytosis.
Earlier Ohe ([2011]) evaluated the educational function of dairy farming with an economic framework as a process of internalization of a positive externality.
In elaborating the external factors for integrative motivation, Deci et al. (1991) propose self-determination theory, which involves a motivated process of internalization, through a continuum from extrinsic towards intrinsic motivation (pp. 328 330).
As the best knowledge known, receptor-mediated endocytosis is a process of internalization of extracellular molecules during which vesicles, for example endosomes and lysosomes, are formed, which is highly characteristic for receptor-mediated endocytosis [35].
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According to that, the factors which catalyse or undermine intrinsic motivation and the processes of internalization and integration may differ between individualistic and collectivistic cultures.
The same applies to extrinsic motivation by fostering or hindering the processes of internalization and integration (Deci and Ryan 1985; Ryan and Deci 2000).
In this study, we developed a simple method to decouple the processes of internalization and phagosomal maturation via three separate measurements, which allowed for a distinction between the time-courses for internalization and acidification, and significantly, provided distinct measurements and thereby comparison of the rates of phagosome acidification and phagosomal-endosomal/lysosomal fusion.
In three separate sets of experiments, we used Alexa488/InlA-beads, FITC/InlA-beads, and a combination of unlabeled InlA-beads with an endosomal/lysosomal dye, to independently measure the separate processes of internalization, phagosomal acidification and phagosomal endosomal/lysosomal fusion, respectively, during phagocytosis by MDCK and Caco-2 cells.
They are embedded in social structures and denote thought systems and processes of "internalization".
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