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At variance with other methodologies, the process of cell isolation is not an issue with this technique because the measurements are conducted at the single-cell level.
We also lack a credible way to prove that the differences between the oscillations of single beta cells and islets were only due to a lack of coupling, rather than loss of some necessary factor in the islet in the process of cell isolation.
Two transcription inhibitors, actinomycin D (50 mg/L, Sigma) and cordycepin (100 mg/L, Sigma), proven effective in suppressing the expression of stress-inducible genes [ 52], were added to all solutions in the process of cell isolation.
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However, as part of our effort to understand the transcriptome of PVD, we are in the process of adopting cell isolation method in C. elegans.
The calvaria were then dissected out and processed for cell isolation and culture.
Number of cells analyzed and number of cell isolations (rats) as indicated.
In this paper we describe the use of a high-throughput process, combining cell isolation, flow cytometry and gene expression profiling to provide physicians with personalized prognostic assessments of multiple myeloma, using bone marrow aspirate, based on the comprehensively validated GEP70 signature.
Furthermore this pre-isolation degradation more or less mimics a prolonged time to storage and initiates the complex process of cell hypoxia and consecutive cell death.
This initiates the process of cell division.
Chondrocytes used for profiling FGFR isoform expression were processed immediately after cell isolation from cartilage.
Evaluating the role of the ATII cell in bovine pulmonary diseases such as BTB requires an efficient method of ATII cell isolation - a process often fraught with challenges.
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