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Specifically, infected astrocyte MTOCs were located to the area of the process in contact with the CTL; at high power the MTOC of both the astrocyte and T cell were found across the immunological synaptic junction (Figures 4, 5).
Evaluation of 216 individual GFP positive cells (4 mice) at high magnification revealed typical radial glia morphology, namely an apical process in contact with the LV and a thin basal fiber that often extended to the pial surface.
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Around 14% of all the dystrophic processes in contact with the amyloid core were dendritic.
These activated astrocytes had many processes in contact with the endothelial cells.
In a wok or when using any continuously stirred process the surfaces in contact with the pan will heat up briefly.
In addition to revealing that the scapular processes are oriented vertically in all basal turtles, the newly prepared specimen of P. robusta demonstrates that the acromion process was nearly in contact with the midline of the plastron (see Additional file 1: Figure S1).
However, in cultures incubated with anti-DCC antibodies, the ApNetrin-1 (green) signal does not colocalize with ApDCC (red) in SN processes that are in contact with the MN (arrowheads) (middle panel, B ).
In cultures in which the MN was microinjected with vehicle, the ApNetrin-1 (green) colocalizes with SN processes that are in contact with the MN soma (arrowheads) (top panel, B ).
In cultures incubated with ASW (top panel, B ) or non-immune IgG (bottom panel, B ), ApNetrin-1 (green) colocalizes with SN processes that are in contact with the MN soma (arrowheads).
Consistent with a previous zebrafish study (Godinho et al., 2005), multipolar iACs appear to stabilize processes that are in contact with the forming IPL (n=50 cells; Fig. 4A; supplementary material Movies 4 and 5).
In cultures in which calcium was chelated in the MN by microinjection of BAPTA, the ApNetrin-1 (green) signal is present within the MN soma, and do not colocalize with the ApDCC (red) in SN processes that are in contact with the MN (arrowheads).
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