How this list is processed for localization is what differentiates the intersection approach from the distance equation approach.
Specimens were embedded in paraffin without decalcification and were processed for localization of MMP28 as described below.
While the VAR1 hotspot was lost, demonstrating that pairing via MHR1 could be an important process for hotspot localization, some hotspots were maintained, thereby indicating the likely involvement of another pairing factor in an MHR1 -independent mechanism.
Sections were processed for immunohistochemical localization of BDNF as described below.
The Fos-labeled sections, also processed for immunocytochemical localization of TH, were rinsed and incubated in 10% NHS in PB for 2 h.
At the end of the RNAi treatment, cells were fixed and processed for Arfip localization using a rabbit anti-Arfip antibody.
The arrays were processed for immunohistochemical localization of CXCR4, using monoclonal anti-human CXCR4 antibodies or IgG2b antibodies for negative control (R&D Systems) as using Vector Elite Kit Vector Laboratoriess, Burlingame, California, USA) as described previously [ 24].
Ca(2+ -dependent retrograde actin flow corrals ER tubule extensions and STIM1/Orai1 complexes to the synapse center, creating a self-organizing process for CRAC channel localization.
Images were processed for co-localization with Metamorph Software v7.0 (Molecular Devices, Downingtown, PA).
At termination, cells are rinsed, fixed with 10% neutral-buffered formalin for 10 min, and stored in 70% ethanol until processed for either histochemical localization of localization of senescence, or immunohistochemical detection of senescence as described above.
Prior to the development of Final Fantasy XIII-2, the standard localization process for a title involved dual development between the company's sound and localization departments, which meant that difficulties could arise because of constant changes to dialogue.
