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We processed paired-end sequence reads (~15X coverage of Illumina's HiSeq 2000) which provided ~15X coverage of the reference pig genome (SusSc.10.2).
In a strange end sequence, the couple returned to Cleese.
Conceptually, the robustness of our results across callers is likely because we are considering effects at a broader length scale (50 kb) than the typical scale of insert sizes (Table 1) in the paired end sequencing process.
The process ended up with 1559 sequences split into two and 50 into three potential transcripts.
For paired-end sequence reads, they are processed to release the end sequences with the pairing information preserved for later use after the linker sequence is identified and removed.
Transcript abundance in silk glands, cephalothorax, and venom glands was estimated by aligning the processed raw paired-end sequence reads from each tissue-specific library to the final non-redundant transcriptome using RSEM [ 63].
Here we demonstrate a process that uses paired-end sequence reads to choose an optimal program and alignment parameters.
BAC end sequencing can be processed in a 96 well format but requires purified DNA template.
The generated 5' and 3' end sequences were pre-processed using a Phred [ 48, 49] cut-off quality value of 20.
The end sequences are then mapped and processed like the single-end reads as described above.
All sequencing reads were collected with standard Sanger sequencing protocols on ABI 3730XL automated sequencers at the Joint Genome Institute, Walnut Creek, CA. Three different sized libraries were used for the plasmid subclone sequencing process and paired-end sequencing.
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CEO of Professional Science Editing for Scientists @ prosciediting.com