In addition, processes that are purely autoregressive are often transformed into ARMA p,p) processes by addition of measurement noise, and especially sinusoids in noise are known to obey the degenerate ARMA equation[8, 9].
In the trans-splicing reaction, long polycistronic messages are processed by addition of a 30 40 nucleotides RNA derived from the spliced leader gene at the 5' end of each cistron, followed by addition of a poly(A) tail at the 3' end.
The RCC cultures were derived using a two-stage process initiated by addition of a small volume of water sample to culture media (e.g., K/5) to stimulate coccolithophore growth and, subsequently, isolate single calcifying cells by micropipette from patches of actively calcifying cells on the bottom of the growth vessel (RCC1200, RCC1203, RCC1214, and RCC1216; I. Probert pers. comm).
The generation of these bubbles is a physical process chemically assisted by addition of surfactants (frothers).
The effect of metakaolin and silica fume on stabilizing air-voids was observed, and the collapse of cement foams during the hardening process was avoided by addition of metakaolin.
The mixtures were incubated at room temperature for 60 min and 105 min, shaking every 5 min to prevent sedimentation of macrophages and microbes, and the phagocytosis process was stopped by addition of 500 µl ice-cold 10 mM PBS (pH 7.4), followed immediately by centrifugation at 200 g at 4°C for 5 min to separate macrophages from non-phagocytosed microbes.
This process was enhanced by addition of TGF-β and partially suppressed by treatment with SB-431542 relative to controls.
Disintegration process was followed by addition of DNaseI (20 U to 1 mL, 15 min incubation at laboratory temperature, Sigma-Aldrich) and sonication (20 W, 6 × 30 s, on ice) with Sonicator® 3000 ultrasonic liquid processor (Misonix Inc).
Aposymbiotic animals were generated by several repetitions of the following process: cold-shocking by addition of 4°C ASW and incubation at 4°C for 4 h, followed by 1 2 days of treatment at ~25°C in ASW containing the photosynthesis inhibitor diuron (Sigma-Aldrich) at 50 μM.
