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TiPED involves a series of tests with ascending sensitivities: computational assessments, high-throughput cellular assays, cell process assays, live-animal testing with fish and amphibians, and, ultimately, mammalian testing.
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We concluded that the changes in the cheese-making process assayed in this work were insufficient to produce significant differences in proteolysis of the cheeses.
The platform does not require any sample preprocessing except dilution, and the process assay time is reported to take ∼30 min per sample.
In the same report, we also gave evidence supporting the association between the over-expression of glucosamine-6-phosphate isomerase (Gln6Pi), the first enzyme in the theoretical route of chitin biosynthesis in amoeba, and the onset of the encystment process assayed by RT-PCR.
Central to record-keeping is the management of documentation related to patients, raw materials, processes, assays and facilities.
Identification of these molecular targets provided pivotal insight into the pathogenic process, diagnostic assays and potential therapeutic strategies [2].
Thus, although some isotigs might be constructed as artifacts of the assembly process, PCR assays demonstrate that contigs and some isotigs can be independently validated.
See ELECTRONIC SUPPLEMENTARY MATERIALS for details. Assay modifications were made throughout the method development process; final assay conditions are described in " Method Validation" below.
The process of assay development requires synthesizing analytical quantities of isotopically-labeled peptides and determining the unique pairs of transitions between parent and product ions of proteotypic peptides with the amino acid sequence non-recurrent in other proteins [2].
Auditors drilled tiny holes into the bars to remove samples that were tested for fineness in a process called assaying.
We have now scaled our TaqMan® RT-PCR screening process to assay 768 wells of data per patient sample.
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