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Therefore, we chose to use total variance in our models instead even though it is a conservative approach that includes process and sampling variance e.g. [ 41, 47].
However, since speciation is an ongoing process and sampling genetic diversity of such lineages can be biased by collection scheme or random factors, it is important to evaluate the robustness of conclusions drawn on individual time samples.
The time scale is necessary for the monitoring of binding process and sampling conformations as previously suggested (Reshetnikov et al., 2011; Zhu et al., 2013).
After biological treatment, the concentrations of E2, E3 and NP decreased remarkably, while E1, EE2 and BPA exhibited varying removal efficiencies that depended on the specific treatment process and sampling season.
Consensus among the main researchers (TC, CA, PW) was reached with regards to the selection process and sampling of participants.
All authors participated in the regeneration process and sampling, and contributed to the final version of the manuscript.
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Further details of the assessment and diagnostic process and sample characteristics have been published previously [ 26].
The recruitment process and sample characteristics have also previously been published [ 1].
Variability arises from both ecological processes and sampling, requiring the modelling of uncertainty, the very nature of statistics [ 1, 2].
However, we did not employ a random sampling process and the sample was from participants who voluntarily attended the courses.
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