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During these moments, as well as a pointless but visually astonishing sequence involving rock monsters, one can't help but get a bit of the old-time religion back in ye bones, even as you ask yourself what the proceeding two hours was about.
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To the third sample, which was used to measure the irreversible binding, an excess of unlabeled toxin was added after one hour of incubation and the reaction was allowed to proceed until two hours.
The interrogation, on and off, had then proceeded for nineteen hours.
Civility and rationality ruled as the rally proceeded for three hours to entertain and inspire Chicagoans to laugh, use their inside voices and stop the madness.
The reaction was allowed to proceed for three hours under vacuum at 90°C, at which time the reactor was purged with dry argon and the quasi-prepolymer was poured into a vessel stored at 4°C.
Excision of the ARS-less cassette was allowed to proceed for four hours.
The ligation reaction was allowed to proceed for four hours before an aliquot was removed and used as the template in a PCR with the 'FrWd-KpnI RF1 -Cbx7' FrWd-KpnI RF1 -Cbx7'rimers.
We proceeded to spend two hours, he in Iowa and I in New York, in what can only be called a state of Intense I.M.
Binding of the anti-NRR antibodies was allowed to proceed for one hour in Tris buffer (25 mM, pH 7.4), containing NaCl (150 mM), CaCl2 (5 mM), 0.05% Tween, and 0.5% BSA.
On one of these late-night strolls, he comforted a student concerned about a loud roommate by saying, "Call me anytime if the problem comes up again and I'll come over". The student proceeded to call every two hours throughout the night not to report that anything had happened, but to ask if it was okay to call if something did.
In the Netherlands the timeframe for DCD to proceed is set at two hours.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com