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However, when detecting different proteins with similar size, stripping buffer (2% SDS, 62.5 mM Tris HCl pH 6.7, 100 mM 2-mercaptoethanol) was used to remove primary and secondary antibodies on the PVDF membrane before proceeding to detect another target.
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Analysis of the albumin protein structure by a circular dichroism (CD) spectroscopy was proceeded to detect the conformational change during a tribological process.
This indicated that students in the high-achievement groups advanced or explained the solutions and then proceeded to detect errors or check the plausibility.
We next proceeded to detect the significantly differentially enriched regions in each of the cancer cell lines, with osteoblast levels as baseline.
In order to evaluate whether M. tuberculosis might persist in adipose tissue in individuals with latent TB, we obtained 57 peri-renal, abdominal and peri-nodal adipose tissue samples from 19 autopsy cases of Mexican individuals who died from causes other than TB, and we proceeded to detect mycobacterial DNA (IS6110) by in situ as well as conventional PCR.
Using the estimated TFA of DosR, we proceeded to detect DosR-regulated genes in a low oxygen environment.
EdU (Click-iT® Labelincorporationies, Life Technologies) incorporation was detected on paraffin sections with Alexa Fluor555 before proceeding to IHC detection.
In our approach, we apply the criteria used by Alesina and Perotti (1995), but proceed backwards to detect all episodes of "successful" debt consolidation in Spain between 1990 and 2010.
The case reported here illustrates the challenges in the timely diagnosis of TRAS, the importance of clinical presentation and the utility of a novel non-contrast renal magnetic resonance angiography using inversion-recovery-prepared balanced steady-state free precession technique (INHANCE®) to detect TRAS before proceeding to angiographic evaluation.
Of the criteria proposed by the expert consensus, dyspnoea, physical findings related to the right heart and WHO functional class were not sufficiently predictive to proceed to the final DETECT algorithm.
To proceed, first we need to detect and filter out the spurious ORF clusters.
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