Exact(1)
Garnering a list of 116 polymorphisms, these were tested with the above criteria before proceeding to assay design.
Similar(59)
These data indicate that Tat-SF1 depletion did not affect T-Rex-293 cell viability, and therefore, we proceeded to assay the role of Tat-SF1 as a cellular cofactor of HIV.
Since blue colonies were indeed observed (data not shown), we proceeded to assay for breakdown of ONPG in crude extracts of H. lacusprofundi.
Nitraria retusa products properties were carried out by firstly evaluating their effects against lipid peroxidation induced by H2O2, using the thiobarbituric acid reactive substances species (TBARS) assay, and proceeding to the assay of cellular antioxidant activity, then doing the comet assay.
The inoculum was incubated at 37°C for 24 hours before proceeding to subsequent assay.
In addition to these receptor binding assays, research is proceeding to develop cell-based assays to identify inhibitors of steroidogenesis, a non-receptor-mediated mechanism of endocrine toxicity (Hilscherova et al. 2004).
The existing literature does not provide detailed information on the various synthesis procedures and characterization techniques before proceeding to the toxicological assays.
On day 9 the cells were washed 3 times in medium and resuspended in 1 ml, and rested over night, before proceeding to a IFNγ ELISPOT assay.
Plasmids were sequenced before proceeding to the dual luciferase assay.
When preincubation of monocytes (10/ml) with human sera was performed, the monocytes were incubated in RPMI 1640 with 50% heat-inactivated (56°C for 30 minutes) serum at 20°C for 30 min. Monocytes were subsequently washed three times in PBS before proceeding to the standard co-culture assay as described above.
A characteristic feature of regulatory T cells is their ability to inhibit cell proliferation of effector T cells, and once we evaluated the role of rSSP4-induced CD4+CD25+FOXP3+ T cells in vivo, we proceeded to perform suppression assays in order to confirm, in vitro, their suppressive capacity.
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