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Without a 'road-map' of how an infection proceeds, it is difficult to identify points of disease intervention or to understand how any single gene product, or family of proteins (from the pathogen or its host), might be important in disease.
Furthermore, as exosome binding and uptake proceed concomitantly, it is difficult to define effects initiated exclusively by binding.
Because the potential proceeds of the rental are small, it is difficult to justify much advertising to attract attention.
Although patients were asked not to proceed to the final question until completing all prior questions, it is difficult to ascertain compliance.
It is difficult to prove this, however, so proceed with caution.
It is difficult to watch.
It is difficult to doubt him.
It is difficult to accept".
It is difficult to know.
It is difficult to say.
It is difficult to understand".
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