Treatments with NEFA and/or insulin were initiated approximately 8 hours later and were allowed to proceed for 24 hours.
The polymerization reaction was left to proceed for 6 hours with continuous stirring (~150 rpm).
"As the hearing is scheduled to proceed for several hours today, we will provide a final update regarding number of arrests at the end of the business day," Eva Malecki, a spokeswoman with the Capitol Police department, said in an email.
Blocking and permeabilization was allowed to proceed for 1 2 hours.
Infection was allowed to proceed for 96 hours before euthanasia.
For experiments using siRNA, differentiation was initiated immediately after the siRNA transfection and allowed to proceed for 48 hours.
HIV vector particles were added to the BER cell lines and infection was allowed to proceed for 6 hours [33].
After washes, infection was allowed to proceed for 16 hours before analysis quantification of CHIKV-positive cells by flow cytometry.
We, therefore, infected HeLa cells with Salmonella (expressing GFP to ease detection of bacteria) and allowed the infection to proceed for 18 hours.
The reaction was allowed to proceed for 16 hours at 20°C after which the product was ethanol purified and dissolved in 100 µl of nuclease free water.
In phosphorylation experiments, following 1 or 2 hours of IPTG induction, 20% L-arabinose was added to a final concentration of 0.2%, and overexpression of the Fyn kinase was allowed to proceed for 2 hours.
