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A-priori the primers and DNA extraction procedures were tested using the amplification conditions described below on L. iners, L. rhamnosus, A. vaginalis and G. vaginalis.
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Differences in patient and procedure characteristics between the 3 countries were tested using the chi-square test.
The repaired frames were tested using the same procedure.
Correlations were tested using the Spearman procedure and a two-tailed p value.
Other ancient DNA and modern DNA libraries were tested using the same procedure.
In addition, untreated biomass and a previously reported pretreatment combination (Entry 1) were tested using the same procedure for comparison.
The statistical significance of differences in measured parameters was tested using the procedures of DPS (Zhejiang University, China).
Presence of null alleles at each locus and sample was tested using the procedure implemented by microchecker with a 99% confidence interval (Van Oosterhout et al. 2004).
The significance of F-statistics was tested using the randomization procedure available in FSTAT using 5000 permutations.
Association of SNPs with HJEB was tested using the CASECONTROL procedure of SAS/Genetics (Statistical Analysis System, version 9.2, Cary, NC, USA, 2010) and PLINK, version 1.07 [22].
Each unique gene pair was tested using the KCCA procedure, resulting in total hypothesis tests.
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