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All the statistical procedures were performed using the STATGRAFICS 5.1 for Windows program (Graphic Software System, STATPOINT TECHNOLOGIES, INC. Warrenton, Virginia, US).
Both procedures were performed using the free software, ImageJ.
Extraction procedures were performed using the Unified Bioaccessibility Method (UBM) to evaluate the bioaccessibility.
All statistical procedures were performed using the Sigma Stat 3.5 statistical package.
All ultrasound procedures were performed using the VEVO-770, fitted with a RMV-706 probe.
The immunostaining procedures were performed using the Benchmark automatic immunostaining device (Ventana Medical System, Tucson, AZ, USA) with affinity-purified goat antibody raised against the 19-amino acid sequence (aa 251 269) of the COOH-terminus of human AQP5 (Alpha Diagnostic, San Antonio, Texas) at a 1∶50 dilution.
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However, several surveys showed that only 50%% of the CVC procedures are performed using the ultrasound-guided technique.
In DK, the majority (95%) of the procedures are performed using the posterior approach (PA).
Due to lack of specialized contrast agents, most fluoroscopic interventional procedures are performed using the same conventional "liquid state" contrast agents before, during, and after the procedure.
Survival time was calculated using the Kaplan-Meier method, and a multivariate analysis of the delays between surgical and radiotherapy procedures was performed using the Cox regression model.
The three fitting procedures are performed using the nonlinear least-squares solver lsqnonlin with a trust-region-reflective algorithm as implemented in the Global Optimization Toolbox of MATLAB.
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