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Standard immunoblotting procedures were followed using the anti-XPress mouse monoclonal antibody (Invitrogen, Cat. no. R910-25) at 1/1000 and the anti-ERK-1 (K2) rabbit polyclonal antibody (Santa Cruz Biotechnology Inc., Cat. no. sc-94) at 1/15,000.
The analysis procedures were followed, using the response variable rated by teacher (TRF) and by mother (CBCL).
Theme and pattern recognition procedures were followed using the Qualitative Analysis Guide of Leuven (QUAGOL) (MM) [ 20].
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The same procedure was followed using the multi-component solution to assess the effect of competing ions.
A thorough data cleaning procedure was followed using the biogeo package in R (M.
A docking procedure was followed using the induced-fit protocol implemented in MOE 2013.08.
Second, the same procedure was followed using the anti-SGH specific antibodies previously enriched using the pull-down strategy instead of the whole sera.
The same procedures were followed using GWAS summary results from the Psychiatric Genomics Consortium available online (https://pgc.unc.edu/).
The same procedures were followed using tissue sections without adding primary antibodies and were used as controls for nonspecific secondary antibody labelling.
For hrs and stam, hrs mutations, similar procedures were followed using females from genotypes hsFLP/+; hrs FRT40A/P[ovoD1],P[ovoD1]FRT40andnd hsFLP/+; stam, hrs FRT40A/P[ovoD1], P[ovoD1]FRT40A, respectively.
Standard western blotting procedures were followed using Tris-glycine SDS-PAGE and electrotransfer onto PVDF membrane at 100 V for 1 h at 4 ºC.
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