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All statistical procedures were conducted using the Statistical Package for the Social Sciences (Version 15.0; SPSS Inc., Chicago, IL, USA).
General molecular biological procedures were conducted using the method described in [ 32].
Initial reverse and 24 h later forward transfection procedures were conducted using the same amounts of siRNAs and transfection reagent.
Geocoding procedures were conducted using the Environmental Systems Research Institute ArcGIS software by Sonoma Technology (Petaluma, CA, USA).
These procedures were conducted using the package "shapes" in R. To estimate the amount of measurement error resulting from digitization, replicate measurements were made on a set of 24 O. excavata individuals and 16 T.
These procedures were conducted using the package "FactoMineR" in R. The observed correlation among the constituent parts of the wing patterns was visualized as a network representation (morphological correlation network).
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All procedures are conducted using the Haemonetics MCS Plus apheresis system.
Construction of the chamber, membrane and experimental procedure were conducted using the recommendations [16].
A whole-body immunohistochemistry procedure was conducted using the tube-fixation protocol according to Wormbook [20].
This procedure was conducted using the STATA command, 'xtlogit', to obtain random-effects logistic regression results.
Formalin-fixed, paraffin-embedded cells were stained for CXCR7 (1:2,000 dilution with antibody 11G8 for detection) using the EnVision FLEX + High pH kit according to the manufacturer's instructions (Dako), and the procedure was conducted using the Autostainer Immunostaining System (Dako).
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