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Survival time was calculated using the Kaplan-Meier method, and a multivariate analysis of the delays between surgical and radiotherapy procedures was performed using the Cox regression model.
DNA preparation for other procedures was performed using the PureGene Genomic DNA Tissue Kit (Qiagen catalog number 158622), following a supplementary Qiagen protocol for nematodes.
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All the statistical procedures were performed using the STATGRAFICS 5.1 for Windows program (Graphic Software System, STATPOINT TECHNOLOGIES, INC. Warrenton, Virginia, US).
Both procedures were performed using the free software, ImageJ.
Extraction procedures were performed using the Unified Bioaccessibility Method (UBM) to evaluate the bioaccessibility.
However, several surveys showed that only 50%% of the CVC procedures are performed using the ultrasound-guided technique.
All statistical procedures were performed using the Sigma Stat 3.5 statistical package.
All ultrasound procedures were performed using the VEVO-770, fitted with a RMV-706 probe.
These procedures were performed using the MEGA4 software [ 53, 54].
All statistical procedures were performed using the BioStat software [ 41].
In DK, the majority (95%) of the procedures are performed using the posterior approach (PA).
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