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The procedures utilized for the preparation of samples for 2-DE are critical to the acquisition of high-quality results for the discovery of biomarkers.
WB was performed on SiHa cell lysates with the same procedures utilized for Phoenix cells.
The procedures utilized for tissue isolation, corticosterone enzyme-linked immunoassay, and ISH have all been described previously [ 54].
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Furthermore, previous studies using polystyrene nanospheres described the vitreous as a barrier to gene transfer [53], however the specialized compaction procedure utilized for the DNA nanoparticles used here produced a significantly smaller particle that seemed to be freely diffusible through the vitreous.
Briefly, the procedure utilized for the array analysis was as follows.
Colonoscopy is a well-established procedure utilized for the evaluation of lower gastrointestinal tract diseases including the screening for colorectal polyps and cancer (CRC) [ 1].
Analysis of current variance as a function of mean current (σ2/I) followed procedures utilized previously for Slo3 [22].
Sensitivity for lateral flexion was 41.2% and for PAS 42.8%, while specificity for lateral flexion was 61.5% and PAS was 62.2% indicating that the motion palpation procedures utilized were neither sensitive nor specific for detecting spinal segmental motion restriction.
A wide range of QC procedures were utilized for this study.
Finally, the optimized procedure was utilized for the analysis of human blood serum reductive (antioxidant) capacity based on amperometric sensing and flow injection analysis at a flow rate of 10 μl/min.
The whole blood procedure was utilized for flow cytometric studies.
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